高受体浓度下结合曲线的解读：计算机分析的实用辅助方法
Interpretation of binding curves obtained with high receptor concentrations: practical aid for computer analysis.
作者信息
Swillens S
机构信息
Institut de Recherche Interdisciplinaire, Université de Bruxelle, Belgium.
出版信息
Mol Pharmacol. 1995 Jun;47(6):1197-203.
PMID:7603460
Abstract
Typical equilibrium binding experiments cannot be quantitatively analyzed on the basis of classical mathematical equations when the receptor concentration is so high that a significant fraction of the added radioligand concentration is in the bound form. In this paper, the appropriate equations are derived and used in a commercial graphics package to estimate the binding parameters, by applying nonlinear regression to pseudo-experimental data. The analysis of saturation and homologous displacement curves obtained with high receptor concentrations reveals that the empirical determination of nonspecific binding by addition of an excess of unlabeled ligand is incorrect.
摘要
当受体浓度过高，以至于添加的放射性配体浓度中有很大一部分处于结合形式时，典型的平衡结合实验无法基于经典数学方程进行定量分析。在本文中，通过对虚拟实验数据应用非线性回归，推导了合适的方程，并将其用于商业图形软件包中以估计结合参数。对高受体浓度下获得的饱和曲线和同源置换曲线的分析表明，通过添加过量未标记配体来凭经验确定非特异性结合是不正确的。
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