Wüthrich B, Stern A, Johansson S G
Department of Dermatology, University Hospital, Zurich, Switzerland.
Allergy. 1995 Feb;50(2):179-83. doi: 10.1111/j.1398-9995.1995.tb05077.x.
A 33-year-old woman without history of previous atopic diseases or drug allergies developed a severe anaphylactic reaction with asthma, vomiting, itching, generalized urticaria, and angioedema during artificial insemination with her husband's sperm. The sperm-processing medium contained bovine serum albumin (BSA). Skin prick test and RAST demonstrated an IgE-mediated hypersensitivity to BSA as well as a polyvalent atopic sensitization to pollens, animal danders, cow's milk, beef, pork, and mutton. SDS-PAGE studies indicated serum albumin to be the appropriate allergen with a high degree of cross-reactivity between serum albumin from different animal species. Artificial insemination with fluid containing potential allergens can, therefore, represent an unnecessary risk for atopic females, even in the absence of prior clinical symptoms of allergic diseases. Preoperative testing with the medium is recommended.
一名33岁女性，既往无特应性疾病或药物过敏史，在接受丈夫精子人工授精期间出现严重过敏反应，伴有哮喘、呕吐、瘙痒、全身性荨麻疹和血管性水肿。精子处理培养基中含有牛血清白蛋白（BSA）。皮肤点刺试验和放射性变应原吸附试验表明，患者对BSA存在IgE介导的超敏反应，同时对花粉、动物皮屑、牛奶、牛肉、猪肉和羊肉存在多价特应性致敏。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳研究表明，血清白蛋白是合适的变应原，不同动物物种的血清白蛋白之间存在高度交叉反应性。因此，即使在没有过敏性疾病既往临床症状的情况下，使用含有潜在变应原的液体进行人工授精对特应性女性来说也可能是不必要的风险。建议术前使用该培养基进行检测。
