Chang C, Jang T
Institute of Biomedical Sciences, Academia Sinica, Taipei, ROC.
Anat Rec. 1995 May;242(1):132-5. doi: 10.1002/ar.1092420117.
Magnetic Resonance Imaging (MRI) has been widely used as a noninvasive diagnostic tool for obtaining morphological, metabolic, and functional information from tissue. However, its potential application in observing detailed structure comparable to that of the light microscope has not yet been fully explored. In order to evaluate the usefulness of MR microscopy, a high resolution three-dimensional (3-D) technique was applied to observe the laminar structure of the mammalian olfactory bulb (OB).
Adult male hamsters (Mesocrecitus auratus) were used as an animal model. Hamster OB and the attached anterior olfactory nucleus were removed from the skull for the MRI examinations. The images were performed with a Bruker AMX-400 system equipped with microimaging accessories. T2 weighted 3-D spin echo sequence was used with a field of view of 9 mm and data matrix of 128128128. The in-plane resolution was 707070 microns. Histological preparation, including vibratome sectioning at 40 microns and Nissl staining, were used for light microscopic evaluations and comparisons.
Five distinct layers from the superficial to the center of the OB were distinguished in the MR images of coronal, sagittal and horizontal slices. As compared to the histological sections at the corresponding cutting planes, the laminar structure of the OB displayed in the MR microscopic images correlated well with its counterparts.
MR microscopy is capable of detecting cellular variation of unsectioned and unstained tissue. It can also be easily applied to obtain spatial information with good resolution. It appears to provide a great potential for diagnostic pathology.
磁共振成像（MRI）已被广泛用作一种非侵入性诊断工具，用于从组织中获取形态学、代谢和功能信息。然而，其在观察与光学显微镜相当的详细结构方面的潜在应用尚未得到充分探索。为了评估磁共振显微镜的实用性，应用了一种高分辨率三维（3-D）技术来观察哺乳动物嗅球（OB）的层状结构。
成年雄性仓鼠（Mesocrecitus auratus）用作动物模型。将仓鼠的嗅球和附着的前嗅核从颅骨中取出进行MRI检查。图像使用配备微成像附件的布鲁克AMX - 400系统进行采集。使用T2加权三维自旋回波序列，视野为9毫米，数据矩阵为128×128×128。平面分辨率为70×70×70微米。组织学制备，包括40微米的振动切片和尼氏染色，用于光学显微镜评估和比较。
在冠状、矢状和水平切片的MR图像中，从嗅球表面到中心可区分出五个不同的层。与相应切割平面的组织学切片相比，MR显微镜图像中显示的嗅球层状结构与其对应物相关性良好。
磁共振显微镜能够检测未切片和未染色组织的细胞变化。它还可以很容易地应用于以良好分辨率获取空间信息。它似乎为诊断病理学提供了巨大潜力。
