Mandaci S, Dobres M S
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104, USA.
Plant Mol Biol. 1997 Aug;34(6):961-5. doi: 10.1023/a:1005804514854.
We describe the isolation, cloning and expression of a 2.8 kb promoter fragment of the Blec4 gene from pea (Pisum sativum cv. Alaska) and demonstrate that it is capable of directing the expression of the beta-glucuronidase coding region to the developing epidermal tissue of vegetative and floral shoot apices of transgenic alfalfa (Medicago sativa cv. RegenSY). The Blec4 promoter represents a useful tool with which to target the expression of foreign genes to the epidermal layer of actively growing shoots. The activity of the Blec4 promoter in the epidermis of the shoot apex makes it particularly suitable for genetically engineering defense against insects and diseases that attack the growing shoot apex.
我们描述了从豌豆（阿拉斯加品种）中分离、克隆和表达Blec4基因2.8 kb启动子片段的过程，并证明它能够将β-葡萄糖醛酸酶编码区的表达导向转基因苜蓿（RegenSY品种）营养和花茎顶端的发育中的表皮组织。Blec4启动子是一种有用的工具，可用于将外源基因的表达靶向到活跃生长的茎的表皮层。Blec4启动子在茎尖表皮中的活性使其特别适合于通过基因工程来抵御攻击生长中的茎尖的昆虫和疾病。
