Applied Biotechnology Research Group, School of Life Sciences, University of Westminster, London, UK.
Eastman Dental Institute, University College London, London, UK.
J Mater Sci Mater Med. 2018 Nov 30;29(12):179. doi: 10.1007/s10856-018-6183-9.
This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20 g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3-hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore confirmed to be biocompatible in nature and suitable for neural regeneration.
本研究验证了野油菜黄单胞菌 CH50（一种野生型菌株）对三酰基甘油(TAGs)的利用，从而生产出具有独特物理性质的新型 mcl-PHA。使用 20g/L 的椰子油可获得 58% 的干重 PHA 产量。化学和结构表征证实，所生产的 mcl-PHA 是一种由三个不同重复单体单元组成的三元共聚物，分别为 3-羟基辛酸酯、3-羟基癸酸酯和 3-羟基十二酸酯或 P(3HO-3HD-3HDD)。考虑到 P(3HO-3HD-3HDD)在生物医学研究中的潜力，特别是在神经组织工程中的潜力，使用 NG108-15（神经元）细胞进行了体外生物相容性研究。细胞活力数据证实 P(3HO-3HD-3HDD)支持 NG108-15 的附着和增殖，因此被确认为具有天然的生物相容性，适合神经再生。
