大肠杆菌K-12中硫酸盐和硫代硫酸盐的转运：硫酸盐结合蛋白与硫代硫酸盐结合蛋白功能重叠的证据
Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins.
作者信息
Sirko A, Zatyka M, Sadowy E, Hulanicka D
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
出版信息
J Bacteriol. 1995 Jul;177(14):4134-6. doi: 10.1128/jb.177.14.4134-4136.1995.
Abstract
In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.
摘要
在大肠杆菌中，硫酸根离子和硫代硫酸根离子由一种ABC型转运体运输，该转运体由膜组分（cysT、cysW和cysA基因的产物）和周质结合蛋白（cysP和sbp基因的产物）组成。单个cysP和sbp突变体能够利用硫酸根和硫代硫酸根作为唯一的硫源，而这两个基因的失活会导致由于两种离子运输受阻而产生半胱氨酸营养缺陷型。
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本文引用的文献
1
CHARACTERIZATION OF A SULFATE- AND THIOSULFATE-TRANSPORTING SYSTEM IN SALMONELLA TYPHIMURIUM.鼠伤寒沙门氏菌中硫酸盐和硫代硫酸盐转运系统的特性研究
J Biol Chem. 1964 Jul;239:2292-7.
2
Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria.细菌细胞外溶质结合受体之间的结构、功能及进化关系。
Microbiol Rev. 1993 Jun;57(2):320-46. doi: 10.1128/mr.57.2.320-346.1993.
3
A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium.硫酸盐的结合位点及其与硫酸盐转运至鼠伤寒沙门氏菌的关系。
J Biol Chem. 1966 Sep 10;241(17):3962-9.
4
Evidence for a sulfate-binding site external to the cell membrane of Salmonella typhimurium.鼠伤寒沙门氏菌细胞膜外存在硫酸盐结合位点的证据。
Biochim Biophys Acta. 1965 Jun 15;104(1):308-10. doi: 10.1016/0304-4165(65)90256-4.
5
Genetics of sulfate transport by Salmonella typhimurium.鼠伤寒沙门氏菌硫酸盐转运的遗传学
J Bacteriol. 1971 Mar;105(3):1053-62. doi: 10.1128/jb.105.3.1053-1062.1971.
6
The effect of triazole on cysteine biosynthesis in Salmonella typhimurium.三唑对鼠伤寒沙门氏菌中半胱氨酸生物合成的影响。
J Gen Microbiol. 1972 Sep;72(2):291-301. doi: 10.1099/00221287-72-2-291.
7
Bacterial periplasmic transport systems: structure, mechanism, and evolution.细菌周质转运系统：结构、机制与进化
Annu Rev Biochem. 1986;55:397-425. doi: 10.1146/annurev.bi.55.070186.002145.
8
The 2 A resolution structure of the sulfate-binding protein involved in active transport in Salmonella typhimurium.鼠伤寒沙门氏菌中参与主动运输的硫酸盐结合蛋白的2A分辨率结构。
J Mol Biol. 1988 Mar 5;200(1):163-80. doi: 10.1016/0022-2836(88)90341-5.
9
Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase.大肠杆菌主要磷酸果糖激酶的核苷酸序列及高效表达
Eur J Biochem. 1985 Jun 3;149(2):363-73. doi: 10.1111/j.1432-1033.1985.tb08934.x.
10
The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.完整大肠杆菌染色体的物理图谱：一种用于大型基因组文库快速分析和分类的新策略的应用
Cell. 1987 Jul 31;50(3):495-508. doi: 10.1016/0092-8674(87)90503-4.
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