Sirko A, Hryniewicz M, Hulanicka D, Böck A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
J Bacteriol. 1990 Jun;172(6):3351-7. doi: 10.1128/jb.172.6.3351-3357.1990.
The nucleotide sequence of the sulfate and thiosulfate transport gene cluster has been determined and located 3' to the gene (cysP) encoding the thiosulfate-binding protein. Four open reading frames, designated cysT, cysW, cysA, and cysM, have been identified. Similarities in primary structure were observed between (i) the deduced amino acid sequences of CysT and CysW with membrane-bound components of other binding protein-dependent transport systems, (ii) that of the CysA sequence with the "conserved" component of such systems, and (iii) that of the CysM sequence with O-acetylserine sulfhydrylase A (cysK gene product) and the beta-subunit of tryptophan synthase (coded by trpB). Expression of the four genes was analyzed in the T7 promoter-polymerase system.
已确定了硫酸盐和硫代硫酸盐转运基因簇的核苷酸序列，并将其定位在编码硫代硫酸盐结合蛋白的基因（cysP）的3'端。已鉴定出四个开放阅读框，分别命名为cysT、cysW、cysA和cysM。在以下方面观察到了一级结构的相似性：（i）CysT和CysW的推导氨基酸序列与其他依赖结合蛋白的转运系统的膜结合成分；（ii）CysA序列与此类系统的“保守”成分；（iii）CysM序列与O-乙酰丝氨酸巯基酶A（cysK基因产物）和色氨酸合酶的β亚基（由trpB编码）。在T7启动子-聚合酶系统中分析了这四个基因的表达。
