寡核糖核酸酶与大肠杆菌中其他已知的外切核糖核酸酶不同。
Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli.
作者信息
Yu D, Deutscher M P
机构信息
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
出版信息
J Bacteriol. 1995 Jul;177(14):4137-9. doi: 10.1128/jb.177.14.4137-4139.1995.
Abstract
Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enzymes, that oligoribonuclease is not a manifestation of RNases D, BN, T, PH, and R, exoribonucleases discovered subsequently. Thus, oligoribonuclease is the eighth distinct exoribonuclease discovered in Escherichia coli. We also show that oligoribonuclease copurifies with polynucleotide phosphorylase.
摘要
寡聚核糖核酸酶是一种对小寡聚核糖核苷酸具有特异性的外切核糖核酸酶，20年前首次得到表征（S. K. 尼约吉和A. K. 达塔，《生物化学杂志》250:7307 - 7312，1975年），并显示与核糖核酸酶II和多核苷酸磷酸化酶不同。在这里，我们使用突变菌株和纯化的酶证明，寡聚核糖核酸酶不是随后发现的核糖核酸酶D、BN、T、PH和R这些外切核糖核酸酶的表现形式。因此，寡聚核糖核酸酶是在大肠杆菌中发现的第八种不同的外切核糖核酸酶。我们还表明，寡聚核糖核酸酶与多核苷酸磷酸化酶共纯化。
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