枯草芽孢杆菌的yvaJ基因编码一种3'至5'外切核糖核酸酶，在缺乏多核苷酸磷酸化酶的菌株中并非必需。
The yvaJ gene of Bacillus subtilis encodes a 3'-to-5' exoribonuclease and is not essential in a strain lacking polynucleotide phosphorylase.
作者信息
Oussenko I A, Bechhofer D H
机构信息
Department of Biochemistry, Mount Sinai School of Medicine of New York University, New York, New York 10029, USA.
出版信息
J Bacteriol. 2000 May;182(9):2639-42. doi: 10.1128/JB.182.9.2639-2642.2000.
Abstract
Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major 3'-to-5' exoribonuclease activity in cell extracts, is viable. Here, we show that the B. subtilis yvaJ gene encodes a second 3'-to-5' exoribonuclease. A strain lacking both of these RNases grows slowly but is viable. The existence of another, as yet unknown, 3'-to-5' exoribonuclease in B. subtilis is suggested.
摘要
对参与信使核糖核酸（mRNA）降解的枯草芽孢杆菌核糖核酸酶（RNases）的研究揭示了一种与大肠杆菌不同的模式。一个缺乏多核苷酸磷酸化酶（细胞提取物中主要的3'至5'外切核糖核酸酶活性）的菌株是可行的。在这里，我们表明枯草芽孢杆菌yvaJ基因编码第二种3'至5'外切核糖核酸酶。一个同时缺乏这两种核糖核酸酶的菌株生长缓慢但仍能存活。这表明在枯草芽孢杆菌中存在另一种尚未知晓的3'至5'外切核糖核酸酶。
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The yvaJ gene of Bacillus subtilis encodes a 3'-to-5' exoribonuclease and is not essential in a strain lacking polynucleotide phosphorylase.枯草芽孢杆菌的yvaJ基因编码一种3'至5'外切核糖核酸酶，在缺乏多核苷酸磷酸化酶的菌株中并非必需。
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