嗜碱假单胞菌H1质粒pAE1易缺失区域的分子特征分析
Molecular characterization of a deletion-prone region of plasmid pAE1 of Alcaligenes eutrophus H1.
作者信息
Chow W Y, Wang C K, Lee W L, Kung S S, Wu Y M
机构信息
Institute of Radiation Biology, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
出版信息
J Bacteriol. 1995 Jul;177(14):4157-61. doi: 10.1128/jb.177.14.4157-4161.1995.
A 93-kb region (D region) of plasmid pAE1 of Alcaligenes eutrophus H1 has been found to have a high rate of spontaneous deletion. In this study, we constructed a restriction endonuclease map and carried out limited sequencing of an approximately 100-kb region from pAE1 which includes the D region (the deleted region) in order to detect and characterize repetitive sequences. Two types of repetitive sequences, the R1 and R2 sequences, were observed to flank the D region; within the D region are three copies of insertion element ISAE1. The R1 and R2 sequences are arranged in direct and inverted orientations, respectively. Molecular analysis of the end product of the deletion is consistent with the hypothesis that the loss of the D-region DNA is the result of recombination between two copies of the R1 sequence. The R1 sequence encodes a 415-amino-acid protein which exhibits substantial sequence similarity to the lambda integrase family of site-specific recombinases. Its genetic function remains to be determined.
已发现嗜碱假单胞菌H1的质粒pAE1的一个93kb区域（D区域）具有较高的自发缺失率。在本研究中，我们构建了限制性内切酶图谱，并对来自pAE1的约100kb区域进行了有限测序，该区域包括D区域（缺失区域），以检测和表征重复序列。观察到两种类型的重复序列，即R1和R2序列，位于D区域两侧；在D区域内有三个插入元件ISAE1的拷贝。R1和R2序列分别以正向和反向排列。缺失终产物的分子分析与以下假设一致，即D区域DNA的丢失是两个R1序列拷贝之间重组的结果。R1序列编码一种415个氨基酸的蛋白质，该蛋白质与位点特异性重组酶的λ整合酶家族具有显著的序列相似性。其遗传功能尚待确定。
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