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两个调控性菌毛基因，fimB和fimE，控制大肠杆菌1型菌毛的相变。

Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli.

作者信息

Klemm P

出版信息

EMBO J. 1986 Jun;5(6):1389-93. doi: 10.1002/j.1460-2075.1986.tb04372.x.

DOI:10.1002/j.1460-2075.1986.tb04372.x
PMID:2874022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1166953/
Abstract

The expression of type 1 fimbriae in Escherichia coli is phase dependent, i.e. a cell is either completely fimbriated or bald. This phenomenon is due to the periodic inversion of a specific 300-bp DNA segment containing the promoter for the fimbrial subunit gene, fimA. The phase switch is controlled by the products of two regulatory genes, fimB and fimE, located upstream of fimA. The fimB and fimE proteins direct the phase switch into the 'on' and 'off' position, respectively. The DNA sequence of a 3000-bp region containing the two genes has been determined. The fimB and fimE proteins exhibit strong homology and have most likely originated by duplication of an ancestral gene. They are highly basic implying that they control the phase switch through interaction at the DNA level.

摘要

大肠杆菌中1型菌毛的表达是相位依赖的，即一个细胞要么完全被菌毛覆盖，要么光秃无毛。这种现象是由于一个特定的300碱基对DNA片段的周期性倒位，该片段包含菌毛亚基基因fimA的启动子。相位转换由位于fimA上游的两个调控基因fimB和fimE的产物控制。fimB和fimE蛋白分别将相位转换引导到“开”和“关”的位置。包含这两个基因的一个3000碱基对区域的DNA序列已被确定。fimB和fimE蛋白表现出很强的同源性，很可能起源于一个祖先基因的复制。它们具有高度碱性，这意味着它们通过在DNA水平上的相互作用来控制相位转换。

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