Eggli P S, Graber W
Institute of Anatomy, University of Bern, Switzerland.
J Histochem Cytochem. 1995 Jul;43(7):689-97. doi: 10.1177/43.7.7608523.
We investigated the distribution of hyaluronan (hyaluronic acid) in rat vascular tissue fixed by an osmium tetroxide (or glutaraldehyde) microwave technique and embedded in epoxy resin (or Lowicryl K4M), using hyaluronan binding proteins coupled to 15-20-nm gold particles as ultrastructural markers in a one-step post-embedding procedure. The intra- and extracellular aspects of vascular endothelial and smooth muscle cell plasma membranes revealed distinct labeling, with a high affinity for caveolae being manifested in both cell types and in all kinds of vessels. Hyaluronan was also localized intercellularly in areas characterized by extensive endothelial cell interdigitation. Intracellularly, moderate staining of nuclear heterochromatin was observed.
我们采用结合了15 - 20纳米金颗粒的透明质酸结合蛋白作为超微结构标记物，在一步包埋后程序中，研究了经四氧化锇（或戊二醛）微波技术固定并包埋于环氧树脂（或Lowicryl K4M）中的大鼠血管组织中透明质酸（玻尿酸）的分布情况。血管内皮和平滑肌细胞质膜的细胞内和细胞外部分均显示出明显的标记，两种细胞类型以及各类血管对小窝均表现出高亲和力。透明质酸也定位于以广泛的内皮细胞交叉指状突为特征的细胞间区域。在细胞内，观察到核异染色质有中度染色。
