Eggli P S, Lucocq J, Ott P, Graber W, van der Zypen E
Institute of Anatomy, University of Bern, Switzerland.
Neuroscience. 1992;48(3):737-44. doi: 10.1016/0306-4522(92)90417-z.
Neural tissue of central (rat spinal cord) and peripheral origin (rat sciatic nerve, nerve fascicles of rat skin and iris and of human conjunctiva) was processed by osmium tetroxide/microwave fixation and embedded in epoxy resin. Hyaluronan-binding proteins and link proteins coupled to 15-20-nm gold particles were used as markers in a one-step post-embedding procedure for identifying hyaluronan (hyaluronic acid) at the ultrastructural level. All myelin sheaths in both rat and human material were found to be intensely labelled. The specificity of the hyaluronan-binding probes was demonstrated by the total loss of labelling following treatment of sections with hyaluronidase or by preincubating either the probes with hyaluronan oligosaccharides or the sections with unlabelled hyaluronan-binding protein. The identified hyaluronan appears to be located extracellularly, but is precise role here remains to be elucidated.
中枢神经组织（大鼠脊髓）和外周神经组织（大鼠坐骨神经、大鼠皮肤、虹膜以及人结膜的神经束）经四氧化锇/微波固定处理后，包埋于环氧树脂中。与15 - 20纳米金颗粒偶联的透明质酸结合蛋白和连接蛋白被用作标记物，在超微结构水平上通过一步后包埋程序鉴定透明质酸。在大鼠和人类组织中，所有髓鞘均被发现有强烈标记。用透明质酸酶处理切片后标记完全消失，或者将探针与透明质酸寡糖预孵育，或将切片与未标记的透明质酸结合蛋白预孵育，均证明了透明质酸结合探针的特异性。所鉴定的透明质酸似乎位于细胞外，但其确切作用仍有待阐明。
