Lee W J, Conroy J, Howell W H, Kocher T D
Department of Zoology, University of New Hampshire, Durham 03824, USA.
J Mol Evol. 1995 Jul;41(1):54-66. doi: 10.1007/BF00174041.
We amplified and sequenced the mitochondrial control region from 23 species representing six families of teleost fish. The length of this segment is highly variable among even closely related species due to the presence of tandemly repeated sequences and large insertions. The position of the repetitive sequences suggests that they arise during replication both near the origin of replication and at the site of termination of the D-loop strand. Many of the conserved sequence blocks (CSBs) observed in mammals are also found among fish. In particular, the mammalian CSB-D is present in all of the fish species studied. Study of potential secondary structures of RNAs from the conserved regions provides little insight into the functional constraints on these regions. The variable structure of these control regions suggests that particular care should be taken to identify the most appropriate segment for studies of intraspecific variation.
我们扩增并测序了来自硬骨鱼六个科的23个物种的线粒体控制区。由于存在串联重复序列和大的插入片段，即使是亲缘关系很近的物种，该片段的长度也高度可变。重复序列的位置表明它们在复制过程中出现在复制起点附近和D环链的终止位点。在哺乳动物中观察到的许多保守序列块（CSB）在鱼类中也有发现。特别是，所研究的所有鱼类物种中都存在哺乳动物的CSB-D。对保守区域RNA潜在二级结构的研究几乎没有揭示这些区域的功能限制。这些控制区的可变结构表明，在确定用于种内变异研究的最合适片段时应格外小心。
