Truyen U, Gruenberg A, Chang S F, Obermaier B, Veijalainen P, Parrish C R
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853, USA.
J Virol. 1995 Aug;69(8):4702-10. doi: 10.1128/JVI.69.8.4702-4710.1995.
A related group of parvoviruses infects members of many different carnivore families. Some of those viruses differ in host range or antigenic properties, but the true relationships are poorly understood. We examined 24 VP1/VP2 and 8 NS1 gene sequences from various parvovirus isolates to determine the phylogenetic relationships between viruses isolated from cats, dogs, Asiatic raccoon dogs, mink, raccoons, and foxes. There were about 1.3% pairwise sequence differences between the VP1/VP2 genes of viruses collected up to four decades apart. Viruses from cats, mink, foxes, and raccoons were not distinguished from each other phylogenetically, but the canine or Asiatic raccoon dog isolates formed a distinct clade. Characteristic antigenic, tissue culture host range, and other properties of the canine isolates have previously been shown to be determined by differences in the VP1/VP2 gene, and we show here that there are at least 10 nucleotide sequence differences which distinguish all canine isolates from any other virus. The VP1/VP2 gene sequences grouped roughly according to the time of virus isolation, and there were similar rates of sequence divergence among the canine isolates and those from the other species. A smaller number of differences were present in the NS1 gene sequences, but a similar phylogeny was revealed. Inoculation of mutants of a feline virus isolate into dogs showed that three or four CPV-specific differences in the VP1/VP2 gene controlled the in vivo canine host range.
一组相关的细小病毒感染许多不同食肉动物科的成员。其中一些病毒在宿主范围或抗原特性上有所不同，但它们之间的真正关系却知之甚少。我们检测了来自各种细小病毒分离株的24个VP1/VP2和8个NS1基因序列，以确定从猫、狗、亚洲貉、水貂、浣熊和狐狸中分离出的病毒之间的系统发育关系。相隔长达40年收集的病毒的VP1/VP2基因之间的成对序列差异约为1.3%。来自猫、水貂、狐狸和浣熊的病毒在系统发育上无法相互区分，但犬类或亚洲貉分离株形成了一个独特的进化枝。先前已证明犬类分离株的特征性抗原、组织培养宿主范围和其他特性是由VP1/VP2基因的差异决定的，我们在此表明，至少有10个核苷酸序列差异将所有犬类分离株与任何其他病毒区分开来。VP1/VP2基因序列大致根据病毒分离时间进行分组，犬类分离株与其他物种分离株的序列分歧率相似。NS1基因序列中的差异较少，但也揭示了类似的系统发育关系。将一种猫病毒分离株的突变体接种到狗身上表明，VP1/VP2基因中三到四个犬细小病毒特异性差异控制了体内犬类宿主范围。
