使用内镜活检标本对结肠癌组织中的核DNA含量进行流式细胞术分析。

Flow cytometric analysis of nuclear DNA content in tissues of colon cancer using endoscopic biopsy specimens.

作者信息

Nishida K, Takano H, Yoneda M, Ohtsuki T, Fujii M, Terasawa Y, Yamane E, Nishioka B, Namura K, Yoshikawa T

机构信息

Department of Gastroenterology, Shakaihoken Kobe Central Hospital, Japan.

出版信息

J Surg Oncol. 1995 Jul;59(3):181-5. doi: 10.1002/jso.2930590309.

DOI:10.1002/jso.2930590309

PMID:7609525

Abstract

Flow cytometric assay of nuclear DNA in endoscopic biopsy specimens was evaluated in colon cancer patients. When the cells were divided into diploid cells and aneuploid cells, aneuploidy was observed in 63% (58 of 92) of the colon cancer patients. However, no clear relation was observed between the frequency of aneuploidy and the invasive depth, size, or histological type of colon cancer. Noncancerous portions of the colon tissues including colon adenoma or normal mucosa were mostly (96%, 87 of 91) diploid. Nuclear DNA content could be analyzed in the fresh biopsy specimens of colon cancer tissues and such investigation might be possibly valuable for further biological characterization of colon cancer in the usual procedure of clinical diagnosis for colonic malignancy before surgical operation or other treatment.

摘要

对结肠癌患者的内镜活检标本进行了细胞核DNA的流式细胞术检测。当细胞分为二倍体细胞和非整倍体细胞时，在63%（92例中的58例）的结肠癌患者中观察到非整倍体现象。然而，未观察到非整倍体频率与结肠癌的浸润深度、大小或组织学类型之间存在明确关系。结肠组织的非癌部分，包括结肠腺瘤或正常黏膜，大多（96%，91例中的87例）为二倍体。可在结肠癌组织的新鲜活检标本中分析细胞核DNA含量，这种研究可能对在手术或其他治疗前的结肠恶性肿瘤临床诊断常规程序中进一步进行结肠癌的生物学特征分析具有价值。