Gaudin-Chazal G, Daszuta A, Faudon M, Ternaux J P
Brain Res. 1979 Jan 12;160(2):281-93. doi: 10.1016/0006-8993(79)90425-6.
Endogenous concentrations of TRP, 5-HT and 5-HIAA were determined in the different cortical and subcortical areas of adult cat's brain, using ion-exchange chromatography and spectrofluorimetric methods. Tryptophan is homogeneously distributed in all structures of the brain. 5-HT is mainly accumulated in the brain stem, hypothalamus and caudate nucleus, but also in cortical areas as olfactory and piriform gyrus. The ratio 5-HIAA/5-HT shows that 5-HT utilization is higher in the structures containing small amounts of 5-HT. Using the radioenzymatic assay described by Saavedra et al. (1973), the rostrocaudal distribution of 5-HT content was determined in olfactory and piriform cortex. Important concentrations of 5-HT were detected in the prepiriformis and anterior amygdaloidea areas. Using the same assay and microdissection on frozen frontal slices of the brain, the distribution of 5-HT concentration throughout the different layers of cortical areas was studied. Piriform, olfactory and sigmoid cortex present high concentrations in the superficial layers, including the molecular one. These results were confirmed by uptake studies, made on crude homogenates from frozen tissue, suggesting that 5-HT is mainly contained in the most external cortical terminals.
采用离子交换色谱法和荧光分光光度法，测定成年猫脑不同皮质和皮质下区域内色氨酸（TRP）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的内源性浓度。色氨酸在脑的所有结构中分布均匀。5-HT主要积聚在脑干、下丘脑和尾状核中，但在嗅觉和梨状回等皮质区域也有积聚。5-HIAA/5-HT的比值表明，在含少量5-HT的结构中，5-HT的利用率较高。采用萨韦德拉等人（1973年）描述的放射酶分析法，测定了嗅觉和梨状皮质中5-HT含量的前后分布。在梨状前区和杏仁前区检测到高浓度的5-HT。使用相同的分析方法并对脑的冷冻额叶切片进行显微切割，研究了5-HT浓度在皮质区域不同层中的分布情况。梨状、嗅觉和乙状结肠皮质在包括分子层在内的表层呈现高浓度。这些结果通过对冷冻组织的粗匀浆进行摄取研究得到了证实，表明5-HT主要存在于最外层的皮质终末中。
