Flemming P, Wellmann A, Maschek H, Lang H, Georgii A
Pathologisches Institut, Medizinische Hochschule Hannover, Germany.
Am J Surg Pathol. 1995 Aug;19(8):927-33. doi: 10.1097/00000478-199508000-00008.
Antibodies against human inhibin, a peptide hormone produced by ovarian granulosa cells to inhibit FSH, are widely applied to determine serum inhibin levels. Recently, they were, however, proved also to stain follicle cells in ovarian tissue by immunoreactions in histological sections. The commercially available inhibin antibody produced by Serotec, applied to sections of paraffin blocks, stained follicle epithelia in 6/6 samples of ovarian tissue from females under the age of 40 recruited from the archives. Adult granulosa cell tumor tissue samples from primary tumors of the ovary showed positive reaction in 6/6 cases. No positive reaction was found in staining tissues from hemangiopericytomas from males (0/3), leiomyomas, leiomyosarcomas, and a malignant melanoma (0/5), serving as negative controls. No positive reactions could be observed in tumor cells of 10 ovarian carcinomas, whereas in two of these cases single cells of the specialized ovarian stroma stained positively with inhibin. Positive immunostainings were revealed in three late metastases (two within the liver) from granulosa cell tumors in females, primarily misinterpreted as hemangiopericytomas or leiomyosarcomas, because the previously resected primaries of the ovary were not known at the time of liver surgery. The recognition of granulosa cell tumors, especially the distinction of the sarcomatoid growth type from soft tissue tumors, may be difficult, even if immunostaining for intermediate filaments are applied. Immunostaining by antibodies against inhibin, which can be applied reliably in histopathology, may therefore provide a useful tool to distinguish between granulosa cell tumors and genuine soft tissue tumors. This is also of clinical importance, because treatment of the former by cisplatin-based polychemotherapy and antisex hormone therapy proved to be helpful. Furthermore, the inhibin antibody can be used as an early serum marker for detecting tumor recurrence months before clinical evidence.
抗人抑制素抗体可广泛用于测定血清抑制素水平，抑制素是一种由卵巢颗粒细胞产生的肽类激素，用于抑制促卵泡激素（FSH）。然而最近发现，通过组织学切片的免疫反应，这些抗体也能使卵巢组织中的卵泡细胞染色。应用Serotec公司生产的市售抑制素抗体对石蜡块切片进行检测，在从档案中收集的40岁以下女性的6份卵巢组织样本中，有6份样本的卵泡上皮细胞被染色。卵巢原发性成年颗粒细胞瘤组织样本在6份病例中均呈阳性反应。作为阴性对照，男性血管外皮细胞瘤（0/3）、平滑肌瘤、平滑肌肉瘤和恶性黑色素瘤（0/5）的染色组织均未发现阳性反应。10例卵巢癌的肿瘤细胞未观察到阳性反应，而其中2例卵巢特殊间质的单个细胞对抑制素呈阳性染色。在女性颗粒细胞瘤的3处晚期转移灶（2处在肝脏）中发现了阳性免疫染色，这些转移灶最初被误诊为血管外皮细胞瘤或平滑肌肉瘤，因为在肝脏手术时不知道先前切除的卵巢原发性肿瘤情况。即使应用中间丝免疫染色，识别颗粒细胞瘤，尤其是将肉瘤样生长类型与软组织肿瘤区分开来可能也很困难。因此，针对抑制素的抗体免疫染色可在组织病理学中可靠应用，可能为区分颗粒细胞瘤和真正的软组织肿瘤提供有用工具。这在临床上也很重要，因为基于顺铂的多药化疗和抗性激素疗法对前者的治疗已证明是有帮助的。此外，抑制素抗体可作为早期血清标志物，在临床证据出现前数月检测肿瘤复发。
