Akaza H, Yokoyama H, Umeda T, Niijima T
Cancer. 1979 Jan;43(1):97-100. doi: 10.1002/1097-0142(197901)43:1<97::aid-cncr2820430115>3.0.co;2-6.
Incubation in vitro of lymphocytes from bladder cancer patients with levamisole (40 microgram/ml) resulted in a rise of the number of E-rosette forming cells from 39.2 +/- 11.8% to 57.5 +/- 11.3% (p less than 0.005). The same effect was observed when levamisole was administrated 150 mg/day for 3 days to the patients. The stimulatory effect of levamisole was abrogated when the lymphocytes were first incubated with levamisole and afterwards with 50% autochthonous serum from the patient. With more diluted serum concentrations (from 0.5% to 1%), the response decreased, and the response was not observed with allogeneic serum. When lymphocytes from healthy donors were incubated with 50% serum from bladder cancer patients, there were no significant changes in numbers of E-rosette forming cells. It was presumed that the suppression of E-rosette forming cells from the patients with bladder cancer was caused by blocking the receptor sites for sheep red blood cells by autochthonous serum components and that levamisole eliminated such substances from the cryptic sites on the surface of the lymphocytes.
将膀胱癌患者的淋巴细胞与左旋咪唑（40微克/毫升）进行体外培养，结果E花环形成细胞数量从39.2±11.8%增至57.5±11.3%（p<0.005）。当给患者每天服用150毫克左旋咪唑，持续3天时，也观察到了同样的效果。当淋巴细胞先与左旋咪唑孵育，然后再与患者50%的自身血清孵育时，左旋咪唑的刺激作用消失。血清浓度稀释得更高（从0.5%至1%）时，反应减弱，而异体血清则未观察到反应。当将健康供者的淋巴细胞与膀胱癌患者50%的血清孵育时，E花环形成细胞数量无显著变化。据推测，膀胱癌患者E花环形成细胞的抑制是由自身血清成分阻断绵羊红细胞的受体位点所致，而左旋咪唑则从淋巴细胞表面的隐蔽位点清除了此类物质。
