Permiakov N K, Anikhovskaia I A, Krupnik A N, Likhoded N V, Likhoded V G, Iakovlev M Iu
Arkh Patol. 1995 Mar-Apr;57(2):4-7.
It is shown that leukocytes binding endotoxin of gram-negative bacteria via Fe-receptor mediated mechanism can be detected in blood smears after the treatment with antibodies to Re chemotype glycolipid conjugated with horse-radish peroxydase. After pretreatment of blood smears by a solution of endotoxin and then by conjugated antibodies to Re glycolipid some leukocytes binding endotoxin in vitro can be detected. It means that some reserves of endotoxin binding by leukocytes can be estimated by this immunomorphological method. The presence of such reserves is characteristic for healthy people and animals. Such reserves were enhanced in animals immunized with heated vaccine from Re Salmonella mutant. The reserves of endotoxin binding by leukocytes are absent in patients with salmonellosis, dysentery, meningitis, viral hepatitis A and B, peritonitis.
结果表明，在用与辣根过氧化物酶偶联的Re化学型糖脂抗体处理后，可在血涂片中检测到通过铁受体介导机制结合革兰氏阴性菌内毒素的白细胞。在用内毒素溶液预处理血涂片，然后用抗Re糖脂结合抗体处理后，可检测到一些在体外结合内毒素的白细胞。这意味着可以通过这种免疫形态学方法评估白细胞结合内毒素的一些储备情况。这种储备的存在是健康人和动物的特征。在用来自Re沙门氏菌突变体的热灭活疫苗免疫的动物中，这种储备增加。沙门氏菌病、痢疾、脑膜炎、甲型和乙型病毒性肝炎、腹膜炎患者不存在白细胞结合内毒素的储备。
