Walker C, Malik R, Canfield P J
Department of Veterinary Pathology, Unviersity of Sydney, New South Wales.
Aust Vet J. 1995 Mar;72(3):93-7. doi: 10.1111/j.1751-0813.1995.tb15017.x.
Although cryptococcosis is a well-characterised disease of cats, the factors predisposing individuals to infection are unknown. As an indication of the immune status of an individual, lymphocyte subsets can be analysed. Reference ranges for feline lymphocyte subsets (Pan T+, CD4+, CD8+ and B cells) were established using a rapid whole blood technique and flow cytometry. There were no effects of age or sex on lymphocyte subset values. The numbers of circulating leucocytes and lymphocyte subsets were determined in FIV-positive and FIV-negative cats with cryptococcosis and compared with a group of healthy control cats. There were only minor differences in the numbers of lymphocyte subsets among the subgroups of cats examined in the study and the predisposition to cryptococcosis in cats could not be explained by deficiencies in lymphocyte subsets. There was a tendency for FIV-negative cats with cryptococcosis to have reduced numbers of circulating CD4+ cells and lower CD4:CD8 ratios compared with normal cats, although the interpretation of this finding was complicated by the wide reference range for normal cats. The extent to which this is the cause of the fungal infection was not determined. The only difference in leucocyte or lymphocytes subset values between FIV-negative cats with cryptococcosis and FIV-positive cats with cryptococcosis was that the CD4+ percentage was lower in the FIV-positive cats. The absolute CD4+ count was similar however, in FIV-positive and FIV-negative cryptococcosis cases. On the basis of this and other available information, the categorisation of cryptococcosis as a disease defining the AIDS phase of FIV infection may be incorrect.
尽管隐球菌病是猫的一种特征明确的疾病，但个体易感染的因素尚不清楚。作为个体免疫状态的一种指标，可以分析淋巴细胞亚群。使用快速全血技术和流式细胞术建立了猫淋巴细胞亚群（全T+、CD4+、CD8+和B细胞）的参考范围。年龄和性别对淋巴细胞亚群值没有影响。测定了患有隐球菌病的FIV阳性和FIV阴性猫的循环白细胞和淋巴细胞亚群数量，并与一组健康对照猫进行比较。在该研究中检测的猫亚组之间，淋巴细胞亚群数量只有微小差异，猫对隐球菌病的易感性不能用淋巴细胞亚群缺陷来解释。与正常猫相比，患有隐球菌病的FIV阴性猫有循环CD4+细胞数量减少和CD4:CD8比值降低的趋势，尽管由于正常猫的参考范围较宽，对这一发现的解释较为复杂。这在多大程度上是真菌感染的原因尚未确定。患有隐球菌病的FIV阴性猫和患有隐球菌病的FIV阳性猫在白细胞或淋巴细胞亚群值上的唯一差异是FIV阳性猫的CD4+百分比更低。然而，在FIV阳性和FIV阴性隐球菌病病例中，绝对CD4+计数相似。基于此及其他现有信息，将隐球菌病归类为定义FIV感染艾滋病阶段的疾病可能是不正确的。
