Reche Archivaldo, Daniel Alexandre G T, Lazaro Strauss Telma C P, Taborda Carlos P, Vieira Marques Shirlei A, Haipek Kátia, Oliveira Lilian J, Monteiro Janaína M, Kfoury José R
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, CEP: 05508-270 São Paulo, Brazil.
J Feline Med Surg. 2010 Apr;12(4):355-8. doi: 10.1016/j.jfms.2009.12.017. Epub 2010 Feb 13.
This study was designed to compare cutaneous mycoflora isolation and CD4+:CD8+ ratio in feline immunodeficiency virus (FIV)-infected cats with that in FIV-uninfected cats. Sixty cats were examined. Twenty-five were FIV-infected cats and 35 were FIV-uninfected cats. All 60 cats were FeLV-negative. Fungi were speciated and immunophenotyping of peripheral CD4+ and CD8+ T lymphocytes was performed. At least one fungal colony was isolated from 22/25 (88%) FIV-infected cats. Among the FIV-uninfected cats fungal colonies were recovered from 13/35 (37%) specimens. Dermatophytes were recovered from 2/25 (8%) FIV-infected cats (one Microsporum gypseum, one Microsporum canis) and 3/35 (8.5%) FIV-uninfected cats (M gypseum). Malassezia species was the most commonly isolated organism from both groups of cats (51.6%). Malassezia species was more commonly isolated from FIV-infected cats than FIV-uninfected cats (84% vs 28.6%). The CD4+ to CD8+ lymphocyte ratio for FIV-infected cats was significantly lower than the CD4+ to CD8+ ratio in the FIV-uninfected cats. The CD4+ to CD8+ lymphocyte ratio for FIV-infected cats with cutaneous overall fungal isolation was significantly lower than the CD4:CD8 lymphocyte ratio in the FIV-infected cats but without cutaneous fungal isolation. We can conclude that immunologic depletion due to retroviral infection might represent a risk factor to cutaneous fungal colonization in cats.
本研究旨在比较感染猫免疫缺陷病毒（FIV）的猫与未感染FIV的猫的皮肤真菌菌群分离情况及CD4⁺:CD8⁺比值。对60只猫进行了检查。其中25只为FIV感染猫，35只为未感染FIV的猫。所有60只猫均为猫白血病病毒（FeLV）阴性。对真菌进行了分类，并对外周血CD4⁺和CD8⁺T淋巴细胞进行了免疫表型分析。22/25（88%）的FIV感染猫至少分离出一个真菌菌落。在未感染FIV的猫中，13/35（37%）的样本中分离出了真菌菌落。2/25（8%）的FIV感染猫分离出皮肤癣菌（一株石膏样小孢子菌，一株犬小孢子菌），3/35（8.5%）的未感染FIV的猫分离出皮肤癣菌（石膏样小孢子菌）。马拉色菌属是两组猫中最常分离出的真菌（51.6%）。马拉色菌属在FIV感染猫中比在未感染FIV的猫中更常被分离出来（84%对28.6%）。FIV感染猫的CD4⁺与CD8⁺淋巴细胞比值显著低于未感染FIV的猫。有皮肤真菌总体分离的FIV感染猫的CD4⁺与CD8⁺淋巴细胞比值显著低于未发生皮肤真菌分离的FIV感染猫的CD4⁺:CD8⁺淋巴细胞比值。我们可以得出结论，逆转录病毒感染导致的免疫耗竭可能是猫皮肤真菌定植的一个危险因素。
