Walker C, Bao S, Canfield P J
Department of Veterinary Pathology, University of Sydney, N.S.W., Australia.
Vet Immunol Immunopathol. 1995 Sep;48(1-2):11-25. doi: 10.1016/0165-2427(95)05421-2.
Two discrete lymphocyte populations were observed commonly on flow cytometric analysis (FCM) of feline lymphocyte subsets. The identity of these populations as small and large lymphocytes was established by correlating data from FCM with that from peripheral blood films. Dual lymphocyte populations were more likely to be seen in feline immunodeficiency virus-positive (FIV(-)+ ve) cats but their occurrence was not affected by health status, age, gender or breed. FIV(-)+ ve cats had a significantly higher proportion of large lymphocytes than FIV-negative (FIV(-)- ve) cats. However, FIV(-)+ ve cats had significantly fewer small lymphocytes than FIV(-)- ve cats but similar numbers of large lymphocytes. Lymphocyte subset analysis revealed that small lymphocytes had a greater proportion of CD4+ cells than large lymphocytes, regardless of the FIV or health status of the cat. In FIV(-)- ve cats, small lymphocytes had a greater proportion of Pan T + lymphocytes than large lymphocytes, but the converse was seen in FIV(-)+ ve cats. The proportion of CD8 + cells was higher in small lymphocytes than large lymphocytes in well FIV(-)- ve cats but this distinction was not seen in sick FIV(-)- ve cats or FIV(-)+ ve cats of any health status. Regardless of health status, FIV(-)+ ve cats had a lower absolute count of small lymphocytes which were T cells (due to lower numbers of both CD4 + and CD8 + cells) than FIV(-)- ve cats. The numbers of small B cells were similar for both FIV(-)+ ve and FIV(-)- ve cats. However, there were no differences between FIV(-)+ ve and FIV(-)- ve cats in the absolute values of any subset of the large lymphocytes, which suggested that FIV may affect only small lymphocytes. Statistically, the inclusion or exclusion of the large lymphocyte population for routine lymphocyte subset analysis did not affect the overall results. However, because there were significant differences in subset distribution between small and large lymphocytes, analysis of both groups should be included in studies examining the role of lymphocytes in disease.
在对猫淋巴细胞亚群进行流式细胞术分析（FCM）时，通常会观察到两个离散的淋巴细胞群体。通过将FCM数据与外周血涂片数据相关联，确定了这些群体分别为小淋巴细胞和大淋巴细胞。双淋巴细胞群体更常见于猫免疫缺陷病毒阳性（FIV（-）+ ve）猫中，但其出现不受健康状况、年龄、性别或品种的影响。FIV（-）+ ve猫的大淋巴细胞比例明显高于FIV阴性（FIV（-）- ve）猫。然而，FIV（-）+ ve猫的小淋巴细胞明显少于FIV（-）- ve猫，但大淋巴细胞数量相似。淋巴细胞亚群分析显示，无论猫的FIV或健康状况如何，小淋巴细胞中的CD4 +细胞比例都比大淋巴细胞高。在FIV（-）- ve猫中，小淋巴细胞中的泛T +淋巴细胞比例比大淋巴细胞高，但在FIV（-）+ ve猫中则相反。在健康的FIV（-）- ve猫中，小淋巴细胞中的CD8 +细胞比例高于大淋巴细胞，但在患病的FIV（-）- ve猫或任何健康状况的FIV（-）+ ve猫中均未观察到这种差异。无论健康状况如何，FIV（-）+ ve猫中作为T细胞的小淋巴细胞绝对计数低于FIV（-）- ve猫（由于CD4 +和CD8 +细胞数量均较低）。FIV（-）+ ve猫和FIV（-）- ve猫的小B细胞数量相似。然而，FIV（-）+ ve猫和FIV（-）- ve猫在大淋巴细胞任何亚群的绝对值上没有差异，这表明FIV可能仅影响小淋巴细胞。从统计学上讲，在常规淋巴细胞亚群分析中纳入或排除大淋巴细胞群体不会影响总体结果。然而，由于小淋巴细胞和大淋巴细胞之间的亚群分布存在显著差异，在研究淋巴细胞在疾病中的作用时，两组分析都应包括在内。
