Moser I, Jobst G, Aschauer E, Svasek P, Varahram M, Urban G, Zanin V A, Tjoutrina G Y, Zharikova A V, Berezov T T
Technical University of Vienna, Institute for Biomedical Microtechniques and Sensorics, Austria.
Biosens Bioelectron. 1995 Summer;10(6-7):527-32. doi: 10.1016/0956-5663(95)96928-r.
Integrated thin film biosensors were developed for the simultaneous measurement of L-glutamine and L-glutamate in a mu-flow cell. Due to a novel glutaminase with an activity optimum in the neutral pH range, direct monitoring of glutamine in a mammalian cell culture medium could be performed. The glutamine bienzyme sensor was prepared by co-immobilization of glutaminase with glutamate oxidase within a photopatterned poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel membrane. The sensor response was linear in the concentration range of 50 mumol to 10 mmol glutamine/l. Additionally, a glutamate biosensor was integrated on the sensor chip for difference measurement of possible glutamate interferences. The sensor-chip could be used for at least 300 measurements without any alteration in the performance of its sensors. A new sensor-chip with an integrated flow cell provided the possibility of simultaneous measurement of four different parameters at a cell volume of 1 microliter. In order to complete the microsystem, and in order to obtain a "lab on chip", a battery operated surface mounted device (SMD) potentiostat was developed.
集成薄膜生物传感器被开发用于在微流池中同时测量L-谷氨酰胺和L-谷氨酸。由于一种在中性pH范围内活性最佳的新型谷氨酰胺酶，能够对哺乳动物细胞培养基中的谷氨酰胺进行直接监测。谷氨酰胺双酶传感器是通过将谷氨酰胺酶与谷氨酸氧化酶共同固定在光图案化的聚甲基丙烯酸2-羟乙酯（pHEMA）水凝胶膜中制备而成。传感器响应在谷氨酰胺浓度为50 μmol至10 mmol/L的范围内呈线性。此外，在传感器芯片上集成了一个谷氨酸生物传感器，用于对可能的谷氨酸干扰进行差值测量。该传感器芯片可用于至少300次测量，而其传感器性能不会有任何改变。一种带有集成微流池的新型传感器芯片提供了在1微升细胞体积下同时测量四个不同参数的可能性。为了完善该微系统，并获得一个“芯片实验室”，开发了一种由电池供电的表面贴装器件（SMD）恒电位仪。
