White S F, Turner A P, Biltewski U, Bradley J, Schmid R D
Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, UK.
Biosens Bioelectron. 1995 Summer;10(6-7):543-51. doi: 10.1016/0956-5663(95)96930-w.
Amperometric biosensors (based on rhodinised carbon electrodes) for glucose, glutamine and glutamate were constructed. The sensors were incorporated into a three cell parallel FIA system and used to monitor the three analytes on-line during two mammalian cell perfusion cultures. All measurements were made simultaneously from undiluted media sample. Use of the FIA system enabled easy and rapid exchange of the sensors, during cultivation. The inclusion of a calibration step, regularly for all sensors, helped to maintain the accuracy of all measurements. Comparison with off-line measurements indicated that all three biosensors operated successfully, providing accurate information.
构建了用于葡萄糖、谷氨酰胺和谷氨酸的电流型生物传感器（基于镀铑碳电极）。这些传感器被整合到一个三通道平行流动注射分析（FIA）系统中，并用于在两种哺乳动物细胞灌注培养过程中在线监测这三种分析物。所有测量均从未稀释的培养基样品中同时进行。在培养过程中，使用FIA系统能够轻松快速地更换传感器。定期对所有传感器进行校准步骤有助于保持所有测量的准确性。与离线测量的比较表明，所有三种生物传感器均成功运行，提供了准确的信息。
