Ward D J, Somkuti G A
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Philadelphia, PA 19118, USA.
Appl Microbiol Biotechnol. 1995 May-Jun;43(2):330-5. doi: 10.1007/BF00172834.
A pH-dependent adsorption/desorption technique was used to screen Streptococcus thermophilus strains for the production of bacteriocins. Agar-diffusion tests with S. thermophilus strains as targets identified 13 out of 41 strains as producers of antibacterial activity. Thermophilin A, the bacteriocin-like substance present in the culture supernatant of S. thermophilus ST134 was purified to homogeneity by ammonium sulfate precipitation and ion-exchange chromatography, followed by ultrafiltration. Thermophilin A is a relatively heat-stable and apparently glycosylated bacteriocin with a bactericidal mode of action against sensitive cells.
采用pH依赖性吸附/解吸技术筛选嗜热链球菌菌株以检测其细菌素的产生情况。以嗜热链球菌菌株为靶标的琼脂扩散试验表明，41株中有13株具有抗菌活性。嗜热链球菌ST134培养上清液中存在的类细菌素物质嗜热菌素A，通过硫酸铵沉淀、离子交换色谱，随后超滤，被纯化至同质。嗜热菌素A是一种相对耐热且明显糖基化的细菌素，对敏感细胞具有杀菌作用模式。
