Ransac S, Blaauw M, Dijkstra B W, Slotboom A T, Boots J W, Verheij H M
Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
J Struct Biol. 1995 Mar-Apr;114(2):153-5. doi: 10.1006/jsbi.1995.1014.
Single cyrstals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space group P212121 with a = 73.31 A, b = 77.96 A, and c = 169.81 A, with two protein molecules per asymmetrical unit. The crystals diffract to at least 2.8 A resolution and are suitable for an X-ray structure analysis.
