Burnet P W, Mead A, Eastwood S L, Lacey K, Harrison P J, Sharp T
University Department of Psychiatry, Radcliffe Infirmary, Oxford, UK.
Neuroreport. 1995 Apr 19;6(6):901-4. doi: 10.1097/00001756-199504190-00019.
We investigated the effect of electroconvulsive shock (ECS), administered five times over 10 days, on 5-HT1A and 5-HT2A receptor mRNA and binding site densities in the rat brain using in situ hybridization histochemistry and quantitative autoradiography. ECS treatment increased 5-HT1A receptor mRNA abundance and binding site densities in the dentate gyrus, but decreased these parameters in the CA3c layer of the hippocampus. No changes in 5-HT1A receptor mRNA and binding sites occurred in other hippocampal subfields, neocortex or raphe nuclei. Repeated ECS was also found to increase 5-HT2A receptor binding site densities in the neocortex and this was accompanied by a non-significant increase in cortical 5-HT2A receptor mRNA abundance. Our study demonstrates that in the rat, repeated ECS produces anatomically and molecularly discrete effects on 5-HT1A and 5-HT2A receptor gene expression. These changes may be relevant to the therapeutic effect of repeated ECS in depression.
我们采用原位杂交组织化学和定量放射自显影技术，研究了在10天内给予5次电惊厥休克（ECS）对大鼠脑内5-羟色胺1A（5-HT1A）和5-羟色胺2A（5-HT2A）受体mRNA及结合位点密度的影响。ECS处理增加了齿状回中5-HT1A受体mRNA丰度和结合位点密度，但降低了海马CA3c层中的这些参数。在其他海马亚区、新皮层或中缝核中，5-HT1A受体mRNA和结合位点未发生变化。还发现重复ECS会增加新皮层中5-HT2A受体结合位点密度，同时皮层5-HT2A受体mRNA丰度有不显著增加。我们的研究表明，在大鼠中，重复ECS对5-HT1A和5-HT2A受体基因表达产生解剖学和分子学上的离散效应。这些变化可能与重复ECS治疗抑郁症的效果有关。
