Nelson J E, Krawetz S A
Dept of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
DNA Seq. 1995;5(3):163-8. doi: 10.3109/10425179509029356.
We have isolated a human cosmid clone encompassing approximately 40 kb of the chromosome 16p13.2 region. This region contains the protamine genes PRM1 and PRM2 and the transition protein gene TNP2. To facilitate the characterization of this under represented region of the chromosome 16 physical map, a detailed map of this cosmid clone was constructed. Analysis revealed that this gene cluster was nestled amid numerous repetitive elements. Under representation of this segment may be attributed to these repetitive sequence elements scattered throughout this region rendering this locus clonally unstable. Verification of the map by cosmid and genomic Southern analysis resolved discrepancies within the literature and showed that this highly repetitive DNA sequence containing cosmid was representative of this region of the human genome. This dynamic and recombinogenic region continues to evolve.
我们分离出了一个人类黏粒克隆，它包含了大约40 kb的16号染色体p13.2区域。该区域包含鱼精蛋白基因PRM1和PRM2以及过渡蛋白基因TNP2。为了便于对16号染色体物理图谱中这个代表性不足的区域进行表征，构建了该黏粒克隆的详细图谱。分析表明，这个基因簇位于众多重复元件之中。该片段的代表性不足可能归因于散布在整个区域的这些重复序列元件，使得这个基因座在克隆上不稳定。通过黏粒和基因组Southern分析对图谱进行验证，解决了文献中的差异，并表明这个包含高度重复DNA序列的黏粒代表了人类基因组的这个区域。这个动态且具有重组活性的区域仍在不断进化。
