犬白细胞介素-2的分离与测序
The isolation and sequence of canine interleukin-2.
作者信息
Dunham S P, Argyle D J, Onions D E
机构信息
Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
出版信息
DNA Seq. 1995;5(3):177-80. doi: 10.3109/10425179509029359.
A cDNA corresponding to canine IL-2 has been isolated and sequenced. The cDNA was synthesised using RT-PCR, with oligonucleotide primers designed from conserved regions of published IL-2 sequences. The cDNA encodes a predicted full length IL-2 protein of 155 amino-acids. At the nucleic acid level, the canine cDNA shows 92, 88, 88, 82 and 74% homology to published sequences of feline, human, equine, bovine and murine IL-2, respectively. The derived protein shows 90, 86, 85, 76 and 75% similarity to feline, human, equine, murine and bovine IL-2 homologs.
已分离并测序了与犬白细胞介素-2（IL-2）对应的cDNA。使用RT-PCR合成该cDNA，寡核苷酸引物是根据已发表的IL-2序列的保守区域设计的。该cDNA编码一个预测的全长155个氨基酸的IL-2蛋白。在核酸水平上，犬cDNA与已发表的猫、人、马、牛和小鼠IL-2序列的同源性分别为92%、88%、88%、82%和74%。推导的蛋白与猫、人、马、小鼠和牛的IL-2同源物的相似性分别为90%、86%、85%、76%和75%。
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