一种用于血清奎尼丁和(3S)-3-羟基奎尼丁的高效液相色谱法。

A high-pressure liquid chromatography method for serum quinidine and (3S)-3-hydroxyquinidine.

作者信息

Weidner N, Ladenson J H, Larson L, Kessler G, McDonald J M

出版信息

Clin Chim Acta. 1979 Jan 1;91(1):7-13. doi: 10.1016/0009-8981(79)90465-0.

Abstract

A bonded reversed phase, high pressure liquid chromatography (HPLC) method for serum quinidine and its major metabolite in serum, (3S)-3-hydroxyquinidine, is presented. The method employs toluene, rather than benzene for extraction of 100 microliter of serum, can utilize either absorbance or fluorescence detectors, and utilizes a column and equipment which is also suitable for theophylline analyses. Quinidine values in sera by the present method correlated well with fluorometric non-chromatographic methods using ethylene chloride (r = 0.932) or benzene-sulfuric acid (r = 0.950) for extraction of the quinidine. The comparison data suggest a therapeutic range for quinidine of 1.3--5.0 mg/liter when measured by HPLC. In over one year of routine use in a clinical chemistry laboratory, the method has proven to be rapid and precise with interassay coefficients of variation of 2.5--5.5%. No interferences with the HPLC method have yet been identified.

摘要

本文介绍了一种用于测定血清中奎尼丁及其主要代谢产物（3S）-3-羟基奎尼丁的键合反相高效液相色谱（HPLC）方法。该方法采用甲苯而非苯来萃取100微升血清，可使用吸光度或荧光检测器，并且所使用的色谱柱和设备也适用于茶碱分析。通过本方法测得的血清中奎尼丁值与使用二氯乙烷（r = 0.932）或苯磺酸（r = 0.950）萃取奎尼丁的荧光非色谱法相关性良好。比较数据表明，通过HPLC测定时，奎尼丁的治疗范围为1.3 - 5.0毫克/升。在临床化学实验室一年多的常规使用中，该方法已被证明快速且精确，批间变异系数为2.5 - 5.5%。尚未发现对HPLC方法有干扰。