通过聚合酶链反应对巴西儿童致病性大肠杆菌菌株进行鉴别
Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR.
作者信息
Tornieporth N G, John J, Salgado K, de Jesus P, Latham E, Melo M C, Gunzburg S T, Riley L W
机构信息
Department of Medicine, Universidade Federal da Bahia, Salvador de Bahia, Brazil.
出版信息
J Clin Microbiol. 1995 May;33(5):1371-4. doi: 10.1128/jcm.33.5.1371-1374.1995.
Abstract
A PCR technique to differentiate pathogenic enteric Escherichia coli strains in a field setting was evaluated. Among 76 children with acute diarrhea, this technique identified 12 children (16%) with enterotoxigenic E. coli, 6 (8%) with enteropathogenic E. coli, and 1 (1%) with enteroinvasive E. coli infection. Compared with the conventional assays, the PCR method proved to be simpler, more rapid, and inexpensive and therefore suitable for application in a developing-country field setting.
摘要
对一种用于在现场环境中区分致病性肠道大肠杆菌菌株的聚合酶链反应（PCR）技术进行了评估。在76名急性腹泻儿童中，该技术识别出12名（16%）产肠毒素大肠杆菌患儿、6名（8%）致病性大肠杆菌患儿和1名（1%）侵袭性大肠杆菌感染患儿。与传统检测方法相比，PCR方法被证明更简单、更快速且成本更低，因此适合在发展中国家的现场环境中应用。
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