Matias-Guiu X, Colomer A, Mato E, Cuatrecasas M, Komminoth P, Prat J, Wolfe H
Department of Pathology, Hospital Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Spain.
J Pathol. 1995 May;176(1):63-8. doi: 10.1002/path.1711760110.
The expression and distribution of ret proto-oncogene mRNA were investigated in five phaeochromocytomas of both familial and sporadic types by in situ hybridization (ISH) using digoxigenin-labelled cRNA probes and Northern blot (NB) analysis with random priming labelled cDNA probes. The probes corresponded to the tyrosine kinase domain of the gene. An excellent correlation was found between the ISH and NB results. By both techniques, the expression of the ret proto-oncogene was detected in three of the five cases, two of four familial tumours, and the only sporadic tumour that was studied. The results confirm that ret is frequently expressed in phaeochromocytomas and suggest that it might be an important event in their development and/or progression.
采用地高辛标记的cRNA探针通过原位杂交（ISH）以及使用随机引物标记的cDNA探针进行Northern印迹（NB）分析，研究了5例家族性和散发性嗜铬细胞瘤中ret原癌基因mRNA的表达及分布情况。这些探针与该基因的酪氨酸激酶结构域相对应。ISH和NB结果之间发现了良好的相关性。通过这两种技术，在5例中的3例、4例家族性肿瘤中的2例以及所研究的唯一1例散发性肿瘤中检测到了ret原癌基因的表达。结果证实ret在嗜铬细胞瘤中经常表达，并提示其可能在嗜铬细胞瘤的发生发展过程中是一个重要事件。
