Tomokuni K
Department of Community Health Science, Saga Medical School.
Nihon Rinsho. 1995 Jun;53(6):1383-8.
Various methods for determining urinary delta-aminolevulinic acid (ALA) have been devised by many investigators since 1956. This paper introduces a history of the methodology in the determination of urinary ALA and application of some methods. These methods can be divided into two groups; one group consists of colorimetric methods based on the color reaction of ALA-pyrrole with Ehrlich's reagent, and the other group consists of fluorometric methods, based on the fluorescence derivatization of ALA and its separation by HPLC. Colorimetric methods are convenient and inexpensive, while these are less specific. On the other hand, the fluorometric HPLC methods are highly sensitive and specific, while these are expensive because of the high cost of the instruments.
自1956年以来，许多研究人员设计了各种测定尿δ-氨基乙酰丙酸（ALA）的方法。本文介绍了尿ALA测定方法的历史以及一些方法的应用。这些方法可分为两组；一组是基于ALA-吡咯与埃利希试剂的显色反应的比色法，另一组是基于ALA的荧光衍生化及其通过高效液相色谱分离的荧光法。比色法方便且成本低，但特异性较差。另一方面，荧光高效液相色谱法高度灵敏且特异，但由于仪器成本高，这些方法价格昂贵。
