采用荧光高效液相色谱法和比色法测定尿中δ-氨基乙酰丙酸（ALA）。

Measurement of urinary delta-aminolevulinic acid (ALA) by fluorometric HPLC and colorimetric methods.

作者信息

Tomokuni K, Ichiba M, Hirai Y

机构信息

Department of Community Health Science, Saga Medical School, Japan.

出版信息

Ind Health. 1992;30(3-4):119-28. doi: 10.2486/indhealth.30.119.

DOI:10.2486/indhealth.30.119

PMID:1301411

Abstract

We devised a fluorometric HPLC method for determining delta-aminolevulinic acid (ALA) in the urine of lead-exposed workers. With this fluorometric HPLC method and the conventional colorimetric method, the concentrations of urinary ALA in 84 lead workers were determined and compared. In the measurement of urinary ALA at lower levels (< or = 5 mg/1), the value of urinary ALA obtained by the fluorometric HPLC method was much lower than that obtained by the conventional colorimetric method, indicating that the colorimetric method also measures urinary ALA-like compounds such as aminoacetone. On the other hand, the measurement of urinary ALA at higher levels (> 5 mg/1) demonstrated that the ALA value obtained by the fluorometric HPLC method corresponded well with that of the conventional colorimetric method. A correlation coefficient between the fluorometric HPLC method and the colorimetric method was 0.856 for 60 urine samples with ALA < or = 5 mg/1, and 0.996 for 24 urine samples with ALA > 5 mg/1.

摘要

我们设计了一种荧光高效液相色谱法，用于测定铅接触工人尿液中的δ-氨基乙酰丙酸（ALA）。采用这种荧光高效液相色谱法和传统比色法，对84名铅作业工人尿液中的ALA浓度进行了测定和比较。在较低水平（≤5mg/1）的尿ALA测量中，荧光高效液相色谱法测得的尿ALA值远低于传统比色法，这表明比色法还能检测尿中类似ALA的化合物，如氨基丙酮。另一方面，在较高水平（>5mg/1）的尿ALA测量中，荧光高效液相色谱法测得的ALA值与传统比色法测得的值相当吻合。对于60份ALA≤5mg/1的尿样，荧光高效液相色谱法与比色法的相关系数为0.856；对于24份ALA>5mg/1的尿样，相关系数为0.996。