Oishi H, Nomiyama H, Nomiyama K, Tomokuni K
Department of Community Health Science, Saga Medical School, Japan.
J Anal Toxicol. 1996 Mar-Apr;20(2):106-10. doi: 10.1093/jat/20.2.106.
A fluorometric high-performance liquid chromatographic (HPLC) method was developed for the highly sensitive measurement of delta-aminolevulinic acid (ALA) in biological materials. By using this method, we determined ALA in the plasma and urine of 418 workers occupationally exposed to lead and in the plasma and urine of 227 controls. The concentrations of ALA in the plasma and urine of lead workers were significantly elevated as compared with those of the controls. The concentration of ALA in plasma and urine was highly correlated with that of lead in blood in lead workers. It was found that the correlation (r = 0.742) between log of plasma ALA concentrations and blood lead concentrations in lead workers was similar to that (r = 0.711) between log of urine ALA concentrations and blood lead concentrations. These results demonstrated that the measurement of ALA in plasma or in urine using a fluorometric HPLC method was useful for the biological monitoring of lead workers.
开发了一种荧光高效液相色谱（HPLC）方法，用于高灵敏度测量生物材料中的δ-氨基乙酰丙酸（ALA）。通过使用该方法，我们测定了418名职业性接触铅的工人的血浆和尿液以及227名对照者的血浆和尿液中的ALA。与对照组相比，铅作业工人血浆和尿液中ALA的浓度显著升高。铅作业工人血浆和尿液中ALA的浓度与血铅浓度高度相关。结果发现，铅作业工人血浆ALA浓度的对数与血铅浓度之间的相关性（r = 0.742）与尿液ALA浓度的对数与血铅浓度之间的相关性（r = 0.711）相似。这些结果表明，使用荧光HPLC方法测量血浆或尿液中的ALA对铅作业工人的生物监测是有用的。
