Non-invasive monitoring of tissue response to radiotherapy by localised proton spin-lattice relaxation time in mice.

Proton spin-lattice relaxation time T1 of the localised tumour portion and its neighbouring portion of tumour-bearing mice legs is successively observed before and after radiotherapy by applying the previously proposed magnetic focusing method. Changes in T1 values are also compared with the histological radiation effects. For un-irradiated tumours, T1 increases gradually with tumour growth. T1 of the tumour portion shows significant decrease after radiotherapy. The decrease in the T1 values of the tumour clearly depends on the single radiation dose of 30, 45 and 60 Gy, respectively. A rapid decrease in the T1 values of the tumour occurs prior to the decrease in tumour volume. These decreases in the T1 values of the tumour after radiotherapy correspond positively to the increase of the histological radiation effects. After the reduction in the T1 values of the irradiated tumour portion, a successive increase in T1 values is observed and suggests local recurrence of the tumour. These results show that localised T1 relaxation time is a good reflection of the tissue response to radiotherapy.