Hör M, Rimpler H, Heinrich M
Institut für Pharmazeutische Biologie, Albert-Ludwigs-Universität, Freiburg, Germany.
Planta Med. 1995 Jun;61(3):208-12. doi: 10.1055/s-2006-958057.
The antisecretory activity of Guazuma ulmifolia bark was examined in rabbit distal colon mounted in an Ussing chamber. Chloride secretion was stimulated by cholera toxin and prostaglandin E2 (PGE2). Guazuma ulmifolia extract (GUE) completely inhibited cholera toxin-induced secretion if the extract was added to the mucosal bath prior to the toxin. Adding the extract after administration of the toxin had no effect on secretion. GUE did not inhibit PGE2-induced chloride secretion. These results indicate an indirect antisecretory mechanism. SDS-PAGE analysis of cholera toxin treated with GUE confirmed this presumption. GUE specifically interacted with the A subunit of the toxin. Preliminary phytochemical examinations showed that the most active fraction contains procyanidins with a degree of polymerisation higher than 8.
在置于尤斯灌流小室中的兔远端结肠中检测了危地马拉榆叶树皮的抗分泌活性。霍乱毒素和前列腺素E2（PGE2）刺激氯离子分泌。如果在毒素之前将危地马拉榆叶提取物（GUE）添加到黏膜浴中，GUE可完全抑制霍乱毒素诱导的分泌。在给予毒素后添加提取物对分泌没有影响。GUE不抑制PGE2诱导的氯离子分泌。这些结果表明存在间接抗分泌机制。对用GUE处理的霍乱毒素进行的SDS-PAGE分析证实了这一推测。GUE与毒素的A亚基特异性相互作用。初步植物化学检查表明，活性最高的部分含有聚合度高于8的原花青素。
