Harris C L, Kolanko C J
Department of Biochemistry, West Virginia University, Robert C. Byrd Health Sciences Center, Morgantown 26506-9142, USA.
Biochem J. 1995 Jul 1;309 ( Pt 1)(Pt 1):321-4. doi: 10.1042/bj3090321.
The size distribution of aminoacyl-tRNA synthetase activity was investigated in cell extracts prepared from Saccharomyces cerevisiae. Bio-Gel A-5M chromatography of 105,000 g supernatants separated isoleucyl-tRNA synthetase activity into three peaks, with apparent molecular masses (Da) of about 100,000, 350,000 and 10(6) or greater. Similar results were obtained with synthetases specific for glutamic acid, serine and tyrosine. Sucrose-density-gradient centrifugation of yeast supernatants also provided evidence for the existence of synthetase complexes. These data provide the first evidence for the existence of a high-molecular-mass aminoacyl-tRNA synthetase complex in yeast, perhaps similar to those reported in higher eukaryotes.
对酿酒酵母制备的细胞提取物中氨酰 - tRNA合成酶活性的大小分布进行了研究。用Bio - Gel A - 5M对105,000g上清液进行层析，将异亮氨酰 - tRNA合成酶活性分离为三个峰，表观分子量（Da）约为100,000、350,000和10⁶或更大。对谷氨酸、丝氨酸和酪氨酸特异性的合成酶也得到了类似结果。酵母上清液的蔗糖密度梯度离心也为合成酶复合物的存在提供了证据。这些数据首次证明酵母中存在高分子量氨酰 - tRNA合成酶复合物，可能类似于在高等真核生物中报道的那些复合物。
