Serrano P A, Rodriguez W A, Pope B, Bennett E L, Rosenzweig M R
Department of Psychology, University of California, Berkeley 94720-1650, USA.
Behav Neurosci. 1995 Apr;109(2):278-84. doi: 10.1037//0735-7044.109.2.278.
Chelerythrine (CHELE), a specific, potent protein kinase C (PKC) inhibitor, disrupts memory formation for a one-trial peck-avoidance task. Three predictions were made about how CHELE, injected into chick brain near the time of training, would affect memory formation, based on previous work with two classes of protein kinase inhibitors (M. R. Rosenzweig et al., 1992; P. A. Serrano et al., 1994) and the in vitro inhibition of PKC by CHELE: (a) CHELE, injected into the intermediate medial hyperstriatum ventrale, would significantly impair memory formation; (b) the amnestic dose would be approximately 10 nmol; (c) CHELE would not produce amnesia for about 45 min after training, but significantly impair memory by 60 min. Experimental tests confirmed each prediction. This study adds to evidence that PKC activity is part of a cascade of neurochemical events initiated by learning and that PKC activity shortly after training is necessary for long-term memory.
白屈菜红碱（CHELE）是一种特异性、强效的蛋白激酶C（PKC）抑制剂，会破坏单次啄击回避任务中的记忆形成。基于之前两类蛋白激酶抑制剂的研究工作（M. R. 罗森茨韦格等人，1992年；P. A. 塞拉诺等人，1994年）以及白屈菜红碱对PKC的体外抑制作用，针对在训练时注射到雏鸡大脑中的白屈菜红碱如何影响记忆形成做出了三项预测：（a）注射到中内侧腹侧上纹状体的白屈菜红碱会显著损害记忆形成；（b）失忆剂量约为10纳摩尔；（c）训练后约45分钟内白屈菜红碱不会产生失忆作用，但在60分钟时会显著损害记忆。实验测试证实了每一项预测。这项研究进一步证明，PKC活性是学习引发的一系列神经化学事件的一部分，并且训练后不久的PKC活性是长期记忆所必需的。
