Göransson H, Vuola J, Linden M, Pätiälä H, Rokkanen P
Department of Orthopaedics and Traumatology, University Central Hospital, Helsinki, Finland.
Arch Orthop Trauma Surg. 1995;114(3):172-8. doi: 10.1007/BF00443392.
In this experimental study with bone defects, we focussed on the one hand on external and internal osteogenic callus formation after filling the defect and on the other on the osteochondrogenic differentiation capacity of 4-day-old fibrous-like callus grafts and 12-day-old woven bone grafts in an osteogenic environment. A standard cortical bone defect of the femur was created in 95 young rats. The defect was filled with a cortical bone graft and 4- and 12-day-old callus grafts. The grafts were transplanted as such or in Nucleopore chambers. Follow-up was done at 1, 2, 3 and 6 weeks. The osteochondrogenic tissue formed was studied histologically and histomorphometrically. The results suggest that the filling of the bone defect had no influence on the primary external and internal osteogenic callus formation at 1 and 2 weeks. At 3 and 6 weeks in the chamber groups the persisting internal bridging woven bone was converted into more compact lamellar bone whereas periosteal callus remained at the edges of the defect. In the other groups at 3 and 6 weeks the normal shape of the cortex was reconstituting. Four-day-old fibrous-like callus formed bone in the Nucleopore chamber, indicating that fibrous-like callus tissue at 4 days contains osteogenic cells. Twelve-day-old callus consisting of woven bone was partially differentiated to cartilage, showing that woven bone contains cells capable of chondrogenic differentiation.
在这项关于骨缺损的实验研究中，一方面，我们关注骨缺损填充后外部和内部成骨痂的形成；另一方面，我们关注4日龄纤维样痂移植物和12日龄编织骨移植物在成骨环境中的骨软骨生成分化能力。在95只年轻大鼠中制造了标准的股骨皮质骨缺损。用皮质骨移植物以及4日龄和12日龄的痂移植物填充缺损。移植物直接移植或置于核孔滤膜小室中移植。在1、2、3和6周进行随访。对形成的骨软骨生成组织进行组织学和组织形态计量学研究。结果表明，骨缺损的填充在1周和2周时对原发性外部和内部成骨痂形成没有影响。在3周和6周时，小室组中持续存在的内部桥接编织骨转变为更致密的板层骨，而骨膜痂仍留在缺损边缘。在其他组中，3周和6周时皮质的正常形态正在重建。4日龄的纤维样痂在核孔滤膜小室中形成骨，表明4日龄的纤维样痂组织含有成骨细胞。由编织骨组成的12日龄痂部分分化为软骨，表明编织骨含有能够进行软骨生成分化的细胞。
