Lee Y S, Wurster R D
Department of Neurological Surgery, Loyola University Medical Center, Maywood, IL 60153, USA.
Cancer Lett. 1995 Jul 13;93(2):157-63. doi: 10.1016/0304-3835(95)03796-y.
The effects of protein synthesis inhibitors (cycloheximide, anisomycin, puromycin and emetine) on the growth of human brain tumor cells were investigated using U-373 MG human astrocytoma and SK-N-MC human neuroblastoma cell lines. These agents inhibited the growth of the tumor cells in a dose-dependent manner. However, these agents did not affect cell viability evaluated by the trypan blue exclusion method, indicating that growth inhibition was due to the inhibition of cell proliferation rather than the induction of cytotoxicity. Anti-proliferation induced by these agents was significantly blocked by the treatments with either free radical scavengers or antioxidants. These results suggest that enhanced oxidative stress may be involved in the anti-proliferation induced by the protein synthesis inhibitors in human brain tumor cells.
使用U-373 MG人星形细胞瘤细胞系和SK-N-MC人神经母细胞瘤细胞系，研究了蛋白质合成抑制剂（环己酰亚胺、茴香霉素、嘌呤霉素和依米丁）对人脑肿瘤细胞生长的影响。这些药物以剂量依赖的方式抑制肿瘤细胞的生长。然而，这些药物并不影响通过台盼蓝排斥法评估的细胞活力，这表明生长抑制是由于细胞增殖的抑制而非细胞毒性的诱导。用自由基清除剂或抗氧化剂处理可显著阻断这些药物诱导的抗增殖作用。这些结果表明，增强的氧化应激可能参与了蛋白质合成抑制剂诱导的人脑肿瘤细胞抗增殖作用。
