百日咳博德特氏菌ptl基因启动子的定位，该基因编码百日咳毒素分泌所必需的蛋白质。
Localization of the promoter for the ptl genes of Bordetella pertussis, which encode proteins essential for secretion of pertussis toxin.
作者信息
Kotob S I, Hausman S Z, Burns D L
机构信息
Division of Bacterial Products, Food and Drug Administration, Bethesda, Maryland 20892, USA.
出版信息
Infect Immun. 1995 Aug;63(8):3227-30. doi: 10.1128/iai.63.8.3227-3230.1995.
Abstract
The ptl locus of Bordetella pertussis, which encodes proteins necessary for the secretion of pertussis toxin into the extracellular medium, is located directly downstream from the ptx locus, which encodes the structural subunits of the toxin. We have found that the ptx promoter is essential for expression of the ptl genes. A strain of B. pertussis which lacked only the ptx promotor region but which retained all other portions of the ptx-ptl region did not produce PtlF. Moreover, insertion of a functional ptx promoter from B. pertussis at the 5' end of the ptx region of Bordetella bronchiseptica resulted in the production of PtlF in B. bronchiseptica, a species which normally does not produce PtlF. These results suggest that the ptx operon is larger than originally proposed and contains both the ptx and ptl genes.
摘要
百日咳博德特氏菌的ptl基因座编码将百日咳毒素分泌到细胞外培养基中所需的蛋白质，它位于ptx基因座的直接下游，ptx基因座编码毒素的结构亚基。我们发现ptx启动子对于ptl基因的表达至关重要。一株仅缺少ptx启动子区域但保留了ptx-ptl区域所有其他部分的百日咳博德特氏菌不产生PtlF。此外，将来自百日咳博德特氏菌的功能性ptx启动子插入支气管败血博德特氏菌ptx区域的5'端，导致通常不产生PtlF的支气管败血博德特氏菌产生了PtlF。这些结果表明ptx操纵子比最初提出的要大，并且包含ptx和ptl基因。
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