Schroeder H R, Yeager F M, Boguslaski R C, Vogelhut P O
J Immunol Methods. 1979;25(3):275-82. doi: 10.1016/0022-1759(79)90115-7.
An immunoassay for thyroxine (T4) monitored by chemiluminescence was evaluated with clinical serums. A thyroxine-label conjugate (T4-L) and serum samples were applied sequentially to alkaline Sephadex G-25 columns which adsorbed the thyroxine species. Other serum components and potential interferents were washed from the column with barbital buffer. Subsequently addition of antibody initiated the binding reaction. After 1 h incubation, the antibody was eluted from the column with a barbital buffer wash. The bound T4-L in the eluate was oxidized in a chemiluminescent detection reaction. The peak light intensity, attained in about 1 sec, was related to T4 concentration by means of standards. The intra-assay precision of the chemiluminescence immunoassay was +/-5% (C.V.). Statistical comparison of T4 levels determined for 28 serums by this method and a reference assay was acceptable (y = 0.95x + 5.9, r = 0.98, Sy square root of y . 100 = 13.1%).
采用临床血清对化学发光监测的甲状腺素（T4）免疫测定法进行了评估。将甲状腺素标记结合物（T4-L）和血清样本依次加到碱性葡聚糖凝胶G-25柱上，该柱可吸附甲状腺素物质。用巴比妥缓冲液从柱上洗去其他血清成分和潜在干扰物。随后加入抗体引发结合反应。孵育1小时后，用巴比妥缓冲液洗脱柱上的抗体。洗脱液中结合的T4-L在化学发光检测反应中被氧化。在约1秒内达到的峰值光强度通过标准品与T4浓度相关。化学发光免疫测定法的批内精密度为±5%（变异系数）。用该方法和参考测定法对28份血清测定的T4水平进行统计学比较，结果可接受（y = 0.95x + 5.9，r = 0.98，Sy/y的平方根×100 = 13.1%）。
