Hurst P R, Gibbs R D, Clark D E, Myers D B
Department of Anatomy and Structural Biology, Medical School, University of Otago, Dunedin, New Zealand.
Reprod Fertil Dev. 1994;6(6):669-77. doi: 10.1071/rd9940669.
Uterine tissues of pregnant rats were extracted to define any changes to the proportions of collagens types I, III and V. The total concentration of extracted collagen was determined in tissue samples from implant and adjacent non-implant (NI) sites. Extracts were also subjected to polyacrylamide gel electrophoresis (PAGE), immunoblotting and gel densitometry to define the collagen types and to determine their relative proportions. By relating the proportions to the collagen concentrations in the extracts, type I was found to be the predominant collagen in both tissue regions although the concentration in the implant sites was lower than that in the NI sites. The concentration of Type I collagen decreased significantly over the period of observation in both implant and NI sites. Although the concentrations of collagen type III and type V also decreased in the implant sites, they did not alter in the NI sites. The results demonstrate that shortly after the initiation of implantation the uterus responds to the presence of the implanting embryo by decreasing the concentration of all three types of collagen. This indicates that their metabolism may, in part, be regulated by similar mechanisms. Furthermore, it was evident that a decrease in the concentration of collagen type I was initiated in uterine areas that, at the time of sampling, were not directly involved with implantation. During the study, it was found that the alpha 1 chain of collagen type V separated into two distinct bands when run on gels containing 3.8 M urea.(ABSTRACT TRUNCATED AT 250 WORDS)
提取怀孕大鼠的子宫组织，以确定I型、III型和V型胶原蛋白比例的任何变化。在来自着床部位和相邻非着床（NI）部位的组织样本中测定提取的胶原蛋白的总浓度。提取物还进行了聚丙烯酰胺凝胶电泳（PAGE）、免疫印迹和凝胶密度测定，以确定胶原蛋白类型并确定其相对比例。通过将这些比例与提取物中的胶原蛋白浓度相关联，发现I型胶原蛋白在两个组织区域中都是主要的胶原蛋白，尽管着床部位的浓度低于NI部位。在观察期内，I型胶原蛋白的浓度在着床部位和NI部位均显著下降。尽管III型和V型胶原蛋白的浓度在着床部位也有所下降，但在NI部位没有变化。结果表明，着床开始后不久，子宫通过降低所有三种类型胶原蛋白的浓度来响应着床胚胎的存在。这表明它们的代谢可能部分受相似机制调节。此外，很明显，I型胶原蛋白浓度的下降在取样时并未直接参与着床的子宫区域就已开始。在研究过程中，发现V型胶原蛋白的α1链在含有3.8 M尿素的凝胶上电泳时会分离成两条明显的条带。（摘要截取自250字）
