Hurst P R, Palmay R D, Myers D B
Department of Anatomy and Structural Biology, Medical School, University of Otago, Dunedin, New Zealand.
Reprod Fertil Dev. 1997;9(4):403-9. doi: 10.1071/r97003.
Uteri of pregnant rats on Days 6, 7 and 8 of pregnancy were studied to determine the histochemical distribution of collagen types III and V and the incorporation of [3H]glycine into fibrillar collagens during the period of embryonic implantation. Types III and V had a similar distribution in the non-decidual stromal region and muscle layers in implantation sites. They were found to have very low levels in the primary decidual tissue on Day 6 and were not detected in developing decidual tissues on Days 7 or 8. Following injection of labelled glycine, collagen was extracted and the specific activity of the collagens determined by fluorography and 3H incorporated into the collagen bands in the gels. It was found that incorporation of label into both types I and III was similar (33.4+/-12.0 and 31.8+/-18.1 cpm microg-1 collagen respectively) but 3.5 times that of type V (7.7+/-5.3 cpm microg-1). These studies suggest that although fibrillar collagens are metabolized or redistributed in the growing decidual tissue, they are incorporated rapidly into the extracellular matrix during remodelling of the outer stroma and muscle tissues.
对妊娠第6、7和8天的孕鼠子宫进行研究，以确定胚胎着床期III型和V型胶原的组织化学分布以及[3H]甘氨酸掺入纤维状胶原的情况。III型和V型胶原在着床部位的非蜕膜基质区和肌层中分布相似。在第6天的初级蜕膜组织中它们的含量非常低，在第7天或第8天发育中的蜕膜组织中未检测到。注射标记甘氨酸后，提取胶原，通过荧光自显影法测定胶原的比活性，并测定掺入凝胶中胶原条带的3H。发现I型和III型胶原的标记掺入量相似（分别为33.4±12.0和31.8±18.1 cpm μg-1胶原），但为V型胶原的3.5倍（7.7±5.3 cpm μg-1）。这些研究表明，虽然纤维状胶原在生长的蜕膜组织中发生代谢或重新分布，但在外部基质和肌肉组织重塑过程中它们迅速掺入细胞外基质。
