亚硒酸盐对离体大鼠肝细胞的毒性、代谢及吸收

Toxicity, metabolism and absorption of selenite by isolated rat hepatocytes.

作者信息

Park Y C, Whanger P D

机构信息

Toxicology Program, Oregon State University, Corvallis 97330, USA.

出版信息

Toxicology. 1995 Jun 26;100(1-3):151-62. doi: 10.1016/0300-483x(95)03080-y.

DOI:10.1016/0300-483x(95)03080-y

PMID:7624872

Abstract

The uptake of various levels of selenite by isolated rat hepatocytes was investigated. The LD50 value of selenite was about 500 microM. The activity of lactic dehydrogenase in the medium was correlated with cell viability as determined by trypan blue exclusion. After incubation of selenite with hepatocytes, protein-bound Se was the predominant form (80-90% of the cellular Se) present. Subcellular fractionation indicated that most of the radioactivity was present in the cytosol when hepatocytes were incubated with 75Se-selenite. The uptake of 75Se by isolated rat hepatocytes was linear with selenite concentration up to the highest amount tested, 200 microM. Sulfite inhibited the uptake of selenite by hepatocytes.

摘要

研究了分离的大鼠肝细胞对不同水平亚硒酸盐的摄取情况。亚硒酸盐的半数致死剂量（LD50）值约为500微摩尔。培养基中乳酸脱氢酶的活性与通过台盼蓝排斥法测定的细胞活力相关。亚硒酸盐与肝细胞孵育后，蛋白质结合的硒是主要存在形式（占细胞硒的80 - 90%）。亚细胞分级分离表明，当肝细胞与75Se - 亚硒酸盐孵育时，大部分放射性存在于细胞质中。分离的大鼠肝细胞对75Se的摄取与亚硒酸盐浓度呈线性关系，直至测试的最高量200微摩尔。亚硫酸盐抑制肝细胞对亚硒酸盐的摄取。