鲍曼不动杆菌喹诺酮耐药临床分离株gyrA基因的突变
Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii.
作者信息
Vila J, Ruiz J, Goñi P, Marcos A, Jimenez de Anta T
机构信息
Departament de Microbiologia, Hospital Clinic, Facultat de Medicina, Universitat de Barcelona, Spain.
出版信息
Antimicrob Agents Chemother. 1995 May;39(5):1201-3. doi: 10.1128/AAC.39.5.1201.
Abstract
The gyrA gene mutations associated with quinolone resistance were determined in 21 epidemiologically unrelated clinical isolates of Acinetobacter baumannii. Our studies highlight the conserved sequences in the quinolone resistance-determining region of the gyrA gene from A. baumannii and other bacteria. All 15 isolates for which the MIC of ciprofloxacin is > or = 4 micrograms/ml showed a change at Ser-83 to Leu. Six strains for which the MIC of ciprofloxacin is 1 microgram/ml did not show any change at Ser-83, although a strain for which the MIC of ciprofloxacin is 1 microgram/ml exhibited a change at Gly-81 to Val. Although it is possible that mutations in other locations of the gyrA gene, the gyrB gene, or in other genes may also contribute to the modulation of the MIC level, our results suggest that a gyrA mutation at Ser-83 is associated with quinolone resistance in A. baumannii.
摘要
在21株流行病学上无关联的鲍曼不动杆菌临床分离株中测定了与喹诺酮耐药性相关的gyrA基因突变。我们的研究突出了鲍曼不动杆菌和其他细菌gyrA基因喹诺酮耐药决定区中的保守序列。环丙沙星MIC≥4微克/毫升的所有15株分离株在第83位丝氨酸处发生了向亮氨酸的改变。环丙沙星MIC为1微克/毫升的6株菌株在第83位丝氨酸处未显示任何改变，尽管环丙沙星MIC为1微克/毫升的1株菌株在第81位甘氨酸处发生了向缬氨酸的改变。虽然gyrA基因、gyrB基因或其他基因其他位置的突变也有可能导致MIC水平的调节，但我们的结果表明，第83位丝氨酸处的gyrA突变与鲍曼不动杆菌的喹诺酮耐药性相关。
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