Hallmann R, Mayer D N, Berg E L, Broermann R, Butcher E C
Max-Planck-Arbeitsgruppen für Rheumatologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Dev Dyn. 1995 Apr;202(4):325-32. doi: 10.1002/aja.1002020402.
Few markers specific for mouse endothelium exist. We describe here one such marker, MECA-32, a monoclonal antibody which shows high specificity for mouse endothelium in both embryonic and mature tissues. The MECA-32 antigen has a M(r) of 50-55 x 10(3) under reducing conditions and M(r) of 100-120 x 10(3) under nonreducing conditions. It is expressed on most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where it has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier. Interestingly, during embryonic development the antigen occurs on the brain vasculature up to day 16 of gestation (E16), whereupon it disappears. The embryonic brain is an avascular organ anlage which is vascularized by ingrowth of external blood vessels. Differentiation of the vasculature to form the blood brain barrier occurs at approximately E16 in the mouse. This differentiation correlates with the downregulation of MECA-32 antigen expression. Between E12 and E16 MECA-32 detects most endothelial cell surfaces of the blood vessels in the brain. No MECA-32 antigen is found in the brain at E17 or any later stage of development with the exception of the vasculature of the circumventricular organs. The results suggest that MECA-32 antigen expression is temporally and spatially correlated with the development of the blood brain barrier.
几乎没有针对小鼠内皮细胞的特异性标志物。我们在此描述一种这样的标志物，即MECA - 32，一种单克隆抗体，它在胚胎组织和成熟组织中对小鼠内皮细胞均显示出高特异性。在还原条件下，MECA - 32抗原的相对分子质量（M(r)）为50 - 55×10³，在非还原条件下为100 - 120×10³。它在胚胎期和成年小鼠的大多数内皮细胞上表达，但脑、骨骼肌和心肌除外，在这些组织中其分布更为局限。在骨骼肌和心肌中，只有小动脉和小静脉表达MECA - 32抗原，而在脑中，其表达与形成血脑屏障的血管分化呈负相关。有趣的是，在胚胎发育过程中，该抗原在妊娠第16天（E16）之前出现在脑血管系统上，随后消失。胚胎脑是一个无血管的器官原基，由外部血管向内生长而血管化。在小鼠中，血管分化形成血脑屏障大约发生在E16。这种分化与MECA - 32抗原表达的下调相关。在E12和E16之间，MECA - 32可检测到脑中血管的大多数内皮细胞表面。在E17或任何发育后期，除了室周器官的血管系统外，脑中未发现MECA - 32抗原。结果表明，MECA - 32抗原的表达在时间和空间上与血脑屏障的发育相关。
