Qin Y, Sato T N
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110, USA.
Dev Dyn. 1995 Feb;202(2):172-80. doi: 10.1002/aja.1002020209.
Molecular mechanisms of endothelial cell differentiation during blood-brain barrier (BBB) development is not well understood due to the lack of specific molecular markers. Here we describe that expression of the mouse multidrug resistance 1a/3 (mdr1a/3) gene can be detected specifically in subsets of vascular endothelial cells associated with neural tissues at as early as embryonic day 10.5 (E10.5). This onset of mdr1a/3 gene expression coincides with the previously described first appearance of morphologically distinct endothelial cells in neural tissues during BBB development. To our knowledge, the mdr1a/3 gene is the earliest endothelial cell differentiation marker gene during BBB development described thus far. In addition, we have found that neither the level nor pattern of mdr1a/3 gene expression in BBB endothelial cells is affected by aberrant cortical neuronal layers in mutant mouse reeler.
由于缺乏特异性分子标志物，血脑屏障（BBB）发育过程中内皮细胞分化的分子机制尚未完全明确。在此我们描述，早在胚胎第10.5天（E10.5），就可在与神经组织相关的血管内皮细胞亚群中特异性检测到小鼠多药耐药1a/3（mdr1a/3）基因的表达。mdr1a/3基因表达的起始与之前描述的血脑屏障发育过程中神经组织中形态学上不同的内皮细胞的首次出现相一致。据我们所知，mdr1a/3基因是迄今为止所描述的血脑屏障发育过程中最早的内皮细胞分化标记基因。此外，我们发现，突变型小鼠reeler中异常的皮质神经元层既不影响血脑屏障内皮细胞中mdr1a/3基因表达的水平，也不影响其表达模式。
